Coding
Part:BBa_K5477012:Design
Designed by: Kate Malana Escobar Group: iGEM24_UCopenhagen (2024-09-26)
NCOA - Nuclear receptor coactivator (Steroid Receptor Coactivator)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1079
Illegal EcoRI site found at 1701
Illegal SpeI site found at 528
Illegal SpeI site found at 829
Illegal SpeI site found at 1896
Illegal PstI site found at 588 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1079
Illegal EcoRI site found at 1701
Illegal NheI site found at 2841
Illegal SpeI site found at 528
Illegal SpeI site found at 829
Illegal SpeI site found at 1896
Illegal PstI site found at 588 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1079
Illegal EcoRI site found at 1701
Illegal BamHI site found at 2343 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1079
Illegal EcoRI site found at 1701
Illegal SpeI site found at 528
Illegal SpeI site found at 829
Illegal SpeI site found at 1896
Illegal PstI site found at 588 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1079
Illegal EcoRI site found at 1701
Illegal SpeI site found at 528
Illegal SpeI site found at 829
Illegal SpeI site found at 1896
Illegal PstI site found at 588 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
In our receptor module, NCOA functions as a coactivator for nuclear receptors like AhR, enhancing their ability to regulate the expression of detoxification genes when activated by ligands such as polycyclic aromatic hydrocarbons (PAHs) or polychlorinated biphenyls (PCBs). This facilitates transcriptional efficiency of AhR upon ligand binding initiating the transcription of NanoLuc in our reporter module.
Source
Homo sapiens